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The operating procedures of fume hoods are the key to ensuring the safe and efficient operation of laboratories. The following is a detailed operating procedure for fume hoods for the reference of laboratory personnel:
Power supply and Lighting:
Check whether the power switch is in the on position.
Turn on the lighting equipment and make sure the light source is normal and the line of sight is clear.
Ventilation system
Confirm that the fume hood is in the exhaust state.
Turn on the fan, observe whether the exhaust state is normal, and listen quietly for any abnormal noises during operation.
Other examinations:
Check whether all the switches and pipelines for water supply and drainage, gas, etc. are normal.
Check whether the interior of the cabinet is clean and free of residues.
Wear personal protective equipment properly, including lab coats, gloves and goggles.
Window adjustment
When conducting experiments, the fume hood window should be half-open (from the lower edge to the user's elbow) to ensure that the user's chest and above are protected by the safety tempered glass of the window.
It must not exceed the "maximum safe operating height" (generally 45 to 50 cm from the lower edge of the window to the table surface).
When no manual operation is required for the experiment, lower the fume hood window to the lowest operating height (generally 10 to 15 cm from the lower edge of the window to the countertop).
Item placement:
The total area of the experimental items placed on the countertop shall not exceed 50% of the countertop panel area.
The items placed should be at least 15 cm away from the inner side of the window to prevent accidents such as being knocked over or overturned.
The items placed should be at least 10 cm away from the rear deflector plate to avoid blocking the air intake of the deflector plate and affecting the exhaust effect.
The equipment should be placed as deep as possible inside the fume hood and at least 15 to 20 centimeters away from the regulating door.
Avoid placing too many devices in the fume hood and keep the air circulating.
Do not leave disposable gloves, tissues, plastic bags, etc. in the fume hood to avoid blocking the exhaust outlet.
Do not stack items unrelated to the experiment. Do not use the fume hood to store reagents.
Experimental operation:
When using chemical reagents, it is essential to pay attention to the safety usage norms to avoid splashing or spilling outside the fume hood.
When using electric heating devices, it is essential to ensure that the temperature and humidity inside the fume hood are at an appropriate level.
When operating with a fume hood, it is not allowed to strike up a conversation, make phone calls or engage in any other activities unrelated to the operation.
It is forbidden to stick your body into the cabinet.
Operations are prohibited within an area less than 15 cm from the inner side of the window.
Power strips or other power sources are strictly prohibited in the fume hood.
The fume hood workbench must not be used for any other functions such as an office desk or a study desk.
When fume hoods are used in the laboratory, ventilation should also be carried out frequently, which is beneficial to the physical health of the test personnel.
When in use, a fume hood should be supplied with air for 10 minutes every 2 hours (i.e., by opening the Windows for ventilation). If the usage time exceeds 5 hours, the Windows should be kept open to prevent negative pressure in the room.
Experiments are prohibited in the fume hood when it is not turned on.
Flammable and explosive items are strictly prohibited from being placed in fume hoods.
When conducting experiments, it is strictly prohibited to store or experiment with flammable and explosive items in the fume hood.
It is prohibited to place mobile power strips or wires in the general cabinet.
Experiments involving the mixture of organic substances and highly chlorinated compounds that are prohibited by the state from being discharged are strictly forbidden in fume hoods.
It is strictly prohibited to place the substances being tested in a fume hood for experiments without safety. Once chemical substances splash out, the source should be cut off immediately.
When moving the upper and lower Windows, do it slowly and gently to avoid your hand being crushed by the door handle.
During the experiment, it is advisable to keep the window 100 to 150mm away from the table surface.
The operation area of the fume hood should be kept unobstructed, and no items should be piled up around the fume hood.
When the fume hood is not in use, the operator should avoid storing too many test equipment or chemical substances on the countertop of the fume hood. Long-term stacking is strictly prohibited.
Turn off the equipment:
After use, the fume hood should be turned off in time, all water and gas switches inside the hood should be turned off, and the power plug outside the hood should be unplugged.
Return the chemical reagents and instruments used in the experiment.
Turn off the fan, the lighting and the power supply.
Cleaning and organizing
Handle the experimental waste properly.
Wipe and clean the countertop.
Lower the window to the lowest position.
Clean the inside and outside of the fume hood to remove any residues.
Chemical substances are prohibited from being stored in fume hoods.
Chemical spill or fire:
Close the window quickly.
Implement corresponding safety measures.
Report to the laboratory safety officer.
In case of fire, the fume hood should be immediately closed and a fire extinguisher should be used to put out the fire, or the police should be called for help.
Fume hood malfunction
Stop using it immediately.
Close the window.
Report the repair in time.
Daily cleaning
Clean the inside and outside of the fume hood in time after each use.
Regularly clean the accumulated dust in all parts of the fume hood.
Regular inspection
Before using the fume hood every day, check whether the ventilation device and the ventilator are working properly.
If any abnormal conditions or noises are found inside the fume hood, report them to the maintenance personnel in time and have them repaired.
Regular maintenance
Carry out cleaning and maintenance every three months, including cleaning and scrubbing all parts of the cabinet (including the inside and outside of the glass window, inner lining plate and deflector plate) with neutral detergent, and lubricating the slide rails, cables and balance pulleys of the regulating door.
A deep maintenance should be carried out every six months, including checking whether the regulating mechanical devices are corroded or damaged, inspecting whether each fan, motor and rotating shaft is operating normally, testing whether each remote control device (faucet, gas cylinder) can operate normally, checking whether there is any leakage in the exhaust outlet pipeline, and checking whether there is any accumulation of impurities inside the exhaust hood.
